Davies, IL
1989
Multidimensional Open-Tubular Column Supercritical Fluid Chromatography Using a Flow-Switching Interface
Davies, I.L.; Xu, B.; Markides, K.E.; Bartle, K.D. and Lee, M.L.
J. Microcol. Sep., 2, 71, 1989. Funded by Gas Research Institute and the Utah Centers of Excellence.
A multidimensional system based on capillary supercritical fluid chromatography (SFC) was constructed that utilizes a simple flow-switching interface between two open-tubular 50-mm i.d. columns. A novel solvent-venting injection technique was incorporated in the system that enables single or multiple 0.5-mL volumes to be injected into an uncoated, yet deactivated, length of capillary precolumn without flooding of the analytical column. The effectiveness of multidimensional capillary SFC (SFC-SFC) for complex mixtures is demonstrated by the analysis of polycyclic aromatic hydrocarbons (PAH) in a coal tar extract, the trace determination of a methylcarbonate pesticide and its metabolites in a bird extract, and a group-type separation of hydrocarbons in a high-boiling petroleum distillate. These examples show for the first time that capillary SFC-SFC is a complementary alternative to other multidimensional chromatographic methods involving liquid or gaseous mobile phases.
Davies, IL
1989
Multidimensional Open-Tubular Column Supercritical Fluid Chromatography Using a Flow-Switching Interface
Davies, I.L.; Xu, B.; Markides, K.E.; Bartle, K.D. and Lee, M.L.
J. Microcol. Sep., 2, 71, 1989. Funded by Gas Research Institute and the Utah Centers of Excellence.
A multidimensional system based on capillary supercritical fluid chromatography (SFC) was constructed that utilizes a simple flow-switching interface between two open-tubular 50-mm i.d. columns. A novel solvent-venting injection technique was incorporated in the system that enables single or multiple 0.5-mL volumes to be injected into an uncoated, yet deactivated, length of capillary precolumn without flooding of the analytical column. The effectiveness of multidimensional capillary SFC (SFC-SFC) for complex mixtures is demonstrated by the analysis of polycyclic aromatic hydrocarbons (PAH) in a coal tar extract, the trace determination of a methylcarbonate pesticide and its metabolites in a bird extract, and a group-type separation of hydrocarbons in a high-boiling petroleum distillate. These examples show for the first time that capillary SFC-SFC is a complementary alternative to other multidimensional chromatographic methods involving liquid or gaseous mobile phases.